It includes bulk substrate recipes, sterilization and inoculation techniques, and tips for managing large numbers of fruiting blocks for commercial operations. This chapter describes the process for creating supplemented sawdust fruiting blocks, which is the preferred method for many growers and many different species of gourmet mushrooms. Chapter 17: Growing Gourmet Mushrooms on Enriched Sawdust It also includes techniques for creating sawdust spawn and propagating liquid cultures. It includes sterile techniques, managing grain transfers and proper storage procedures. This chapter covers the process of creating and expanding grain spawn, starting from a mushroom culture on agar to thousands of punds of grain spawn. Techniques such as cloning from mushroom tissue and germinating spores are covered in great detail. Included are 5 different recipes for agar and suggested supplements to add to the agar for improved mycelia growth. This is a crucially important skill for the cultivator to hone, yet it is often the most difficult to grasp. This chapter discusses tools and techniques for propagating mushroom culture on agar. Chapter 12: Culturing Mushroom Mycelium on Agar
Different techniques are discussed for reducing outbreaks of contamination, with the theory that it is much easier to prevent contamination than it is to remove it from your operation once it has taken hold somewhere. These are the cultivator, the air, the media, the tools, the innoculum and mobile contamination units (such as flys). Paul outlines the six places you should concentrate on to try and eliminate sources of contamination. Chapter 10: The Six Vectors of Contamination
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